Biologist

Job Title: Biologist
Location: Boston, MA
Contractor Work Model: Onsite – onsite
Hours: M-F 9am-5pm
Compensation: $48.69 - $57.28/hr
Type: Contract, Potential for extension or conversion to permanent

Overview
As a Scientist in Cell Engineering and Assay Development, you will engineer novel cell lines and develop the in vitro assays used to characterize them in support of our allogeneic cell therapy programs. You will design genetic constructs and mRNA, generate engineered cell lines using viral vectors and transient mRNA-based expression, and build cell-based assays to evaluate the function of engineered cells. You will work closely with cross-functional teams to advance cell therapy programs from discovery through preclinical development.

Responsibilities

• Design and engineer novel cell lines in iPSCs, tumor cell lines, and other immortalized and primary cell types to support allogeneic cell therapy programs
• Generate stable engineered cell lines using lentiviral transduction and other viral vector systems
• Design genetic constructs and mRNA for stable and transient gene expression, including knock-in, knockout, and reporter strategies
• Perform transient mRNA-based expression to rapidly prototype and validate cell engineering strategies
• Establish clonal engineered cell lines through cell sorting (FACS) and single-cell cloning
• Develop, optimize, and execute cell-based assays to characterize engineered cells, including live-cell analysis on the Incucyte platform
• Design and run in-depth, multi-parameter flow cytometry panels to phenotype engineered cells and assess immune checkpoint receptor expression
• Evaluate the functional impact of genetic modifications on engineered cell phenotype and function
• Design and execute experiments, analyze data, and interpret results to evaluate the performance of engineered cells in vitro
• Communicate experimental data effectively through written reports and oral presentations.
• Demonstrate flexibility and motivation to learn and apply new technologies for cell engineering and assay development.

• Bachelor’s degree in Cell Biology, Molecular Biology, Immunology, or a related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research.
• Master’s degree in Cell Biology, Molecular Biology, Immunology, or a related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research.
• Hands-on experience with lentiviral cell line generation is required.

• Experience engineering iPSCs and tumor cell lines
• Proficiency in construct design and mRNA design
• Experience with cell engineering using viral vectors and transient mRNA-based expression
• In-depth flow cytometry expertise, including panel design, cell sorting (FACS), and multi-parameter data analysis
• Working knowledge of immune checkpoint receptor biology
• Experience with live-cell analysis platforms such as Incucyte and xCELLigence
• Proficient in developing in vitro cell-based assays, including work with human cell lines and primary cells
• Strong analytical, problem-solving, and decision-making skills.
• Excellent interpersonal and communication skills.
• Ability to work independently and collaboratively within a team environment.

System One, and its subsidiaries including Joulé and Mountain Ltd., are leaders in delivering outsourced services and workforce solutions across North America. We help clients get work done more efficiently and economically, without compromising quality. System One not only serves as a valued partner for our clients, but we offer eligible employees health and welfare benefits coverage options including medical, dental, vision, spending accounts, life insurance, voluntary plans, as well as participation in a 401(k) plan.

System One is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, age, national origin, disability, family care or medical leave status, genetic information, veteran status, marital status, or any other characteristic protected by applicable federal, state, or local law.

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Ref: #558-Scientific