Asepsis, Biology, Cell Biology, Cell Cultures, Data Analysis, Detail Oriented, Droplet Digital PCR (ddPCR), Equipment Maintenance/Repair, Gene Therapy, Laboratory, Molecular Biology, Polymerase Chain Reaction (PCR), Process Development, Production Support, Research Laboratory, Wireless Protocols/Standards
Cell line Biologist requires:
- Prior research experience with protein expression systems
- Experience in HEK293 and/or Sf9 cell culture systems
- Viral vector gene therapy experience, preferably AAV
- Bachelors with 2+ years/Masters with 0+ years of laboratory research experience in academia/industry
- Solid understanding of principles of common molecular and cellular biology protocols and techniques
- Experience performing basic techniques in molecular biology and cell biology, such as cell culture, aseptic technique, transient transfection, ddPCR
- Detail oriented, with experience handling high-value biological samples
Duties;
- Execute on AAV batch production to support nonclinical research needs
- Passage and split cells regularly for use in various lab studies.
- Perform PCR on samples generated by the Process Development team.
- Prepare aliquots for study samples and manage the -80C/LN2 freezer inventory.
- Keep detailed notebooks in ELN and reports describing experimental methods/results.
- Manage the maintenance of equipment and instruments.
- Critically assess data generated from diverse experimental approaches in cell and molecular biology