Research Technician B

The laboratory of Dr. Ian Willis is seeking a highly motivated research technician to support basic research and preclinical studies of gene expression and protein function using engineered mouse models with enhanced health span or neurodegenerative disease.  The work is focused on the RNA polymerase (Pol) III system, its production of RNA molecules at the center of the Central Dogma of Molecular Biology and the health and disease impacts of mutations in this machinery. We employ genetics, biochemistry, cell and molecular biology and omics technologies in mouse models to understand disease pathogenesis and to test potential therapeutic approaches aimed at improving patient care and enhancing health.  

The research technician will support basic research and preclinical studies of gene expression and protein function using engineered mouse models with enhanced health span or neurodegenerative disease. 

• Perform a moderate number of simple or moderate technical procedures;
  • Mouse Colony Management
  • Mouse genotyping, tail tipping and PCR assays
  • Immunohistochemical staining of sectioned tissue and image acquisition by fluorescence microscopy
  • Harvesting and processing of mouse tissue for histology, RNA analysis, proteomics and related procedures
  • Preparation of protein extracts from frozen tissue and western blotting
• Require occasional guidance;
• Occasionally be responsible for ordering routine supplies;
• Rarely modify technical procedures;
• Rarely train other lab members;
• Rarely guide other lab members;
• A basic understanding of scientific literature and of the research goals and rationale is required and will be part of ongoing training in the laboratory;
• Analyze data after instruction
• Interpret data after instruction;
• Rarely make written contributions to papers and grant proposals.*

• Required to have at least a Bachelor’s Degree in a related science (biological science is preferred) or equivalent skills and related experience, and zero to two years of related experience.
• Undergraduate or postbaccalaureate experience in a biological sciences laboratory is preferred