Research Technician III, Cellular and Molecular Medicine (Part-Time)

Perform complex Cell Biology, Molecular Biology, and Biochemistry techniques with minimal supervision including but not limited to:

• Mammalian cell culture
• Cell biological assays
• DNA/RNA isolation
• Molecular cloning
• qPCR
• Western blotting
• Protein extraction and purification
• Immunofluorescence microscopy

Maintain mouse colonies which includes:

• Breeding and weaning
• Genotyping
• Harvesting tissues
• Performing physiological assessments

Protocol Design and Refinements

• With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments.
• Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs).

Record-keeping, Analysis, and Presentations

• Communicates effectively all aspects of experiments with PI or other laboratory staff.
• Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases.
• Catalogs specimens and samples.
• Performs independent analysis and interpretation of data for investigator .

Operational Support

• Orders and catalogs laboratory supplies and supports laboratory operations.
• Operating, maintaining and troubleshoot highly specialized and sophisticated scientific apparatuses, equipment, and instruments.

Knowledge, Skills, and Abilities:

• Aptitude for quick learning and multi-tasking.
• Knowledge of cell biology, molecular biology, and biochemistry principles and techniques.
• Expertise in cell culture, molecular cloning, nucleic acid analysis, protein biochemistry, and microscopy techniques.
• Skill in troubleshooting laboratory procedures and optimizing experimental protocols.
• Experience handling laboratory mice.
• Strong organizational, communication, and project management skills.
• Ability to work independently while contributing effectively within a collaborative research team.