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Job Summary
The Bjorkman Lab in the Division of Biology and Biological Engineering is seeking a motivated individual to join as a Research Technician. The successful candidate will assist a multidisciplinary research team on one or more projects to study antibody response and immunization strategies as part of an effort to develop novel vaccines and antibody therapeutics against viral pathogens.
Essential Job Duties
Molecular biology tasks, including cloning (PCR, gel electrophoresis, sequencing, and validating constructs, etc.) and plasmid preparation.
Transfection and maintenance of mammalian and yeast cell lines.
Protein purification (affinity and size-exclusion chromatography).
Western blotting and SDS-PAGE.
Bioconjugations of proteins, ELISAs, and other assays.
Flow cytometry and/or cell sorting.
General lab duties, including buffer and reagent prep.
Maintaining records.
Other duties as assigned.
Basic Qualifications
BA or BS in Biology or related field, or an educational emphasis in Biology or Chemistry, required by date of hire.
At least one year of hands-on wet lab experience in a biology or chemistry research laboratory.
Must understand the basic workflow and operations of laboratory research processes.
Must have good practical experience in all basic molecular biology techniques, especially the techniques related to the essential job duties.
Experience with recombinant expression of proteins.
Experience with purification of proteins (e.g., affinity, size exclusion, etc.).
Mastery of buffer prep and calculations.
Excellent organization and communication skills (oral and written).
Ability to work in a team environment.
Preferred Qualifications
A background in immunology, molecular biology, or structural biology.
Flow cytometry experience.
Experience working with yeast and/or phage display systems.
BSL-2 experience.
Transfection and maintenance of mammalian or yeast cell lines.
Required Documents
Resume
Cover Letter
Numbers & Facts
Location
Pasadena, CA
Skills
Antibodiesunmatched
Assaysunmatched
Bioengineeringunmatched
Biologyunmatched
Chemistryunmatched
Chromatographyunmatched
Cloningunmatched
Communication Skillsunmatched
Cross-Functionalunmatched
Immunologyunmatched
Laboratoryunmatched
Laboratory Operationsunmatched
Molecular Biologyunmatched
Operations Researchunmatched
Organizational Skillsunmatched
Pathogensunmatched
Polymerase Chain Reaction (PCR)unmatched
Presentation/Verbal Skillsunmatched
Protein Purificationunmatched
Record Keepingunmatched
Research Laboratoryunmatched
Team Playerunmatched
Vaccinationunmatched
Writing Skillsunmatched
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