Scientist I (Gene Therapy/ Cell Cloning)

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” <\/b>based out of San Diego, CA.<\/b><\/span>
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Job Duration: Long Term Contract (Possibility Of Extension)<\/span><\/b>
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Pay Rate : $46.43/hr on W2<\/span><\/b>
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Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K<\/span><\/b>
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The Scientist I will be a vital contributor to our non -viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro -transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT -PCR, and luciferase reporter assays.<\/span>
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Job Responsibilities:<\/span><\/u><\/b>
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·       <\/span><\/span><\/span>Design and clone expression vectors and IVT donor plasmids to support research initiatives.<\/span>
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·       <\/span><\/span><\/span>Execute DNA and mRNA transfections in mouse and human cell lines.<\/span>
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·       <\/span><\/span><\/span>Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.<\/span>
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·       <\/span><\/span><\/span>Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides<\/span>
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Required Qualifications:<\/span><\/u><\/b>
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• Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.<\/span>
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• Molecular Biology:<\/span><\/u> Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site -directed mutagenesis) and IVT.<\/span>
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• Functional Assays:<\/span><\/u> Hands -on expertise in measuring transgene delivery success via reporter assays and qRT -PCR.<\/span>
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• Cell Culture:<\/span><\/u> Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).<\/span>
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• Desired proficiency in Snapgene, Prism, and MS Office.<\/span>
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  If interested, please send us your updated resume at<\/span>
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  hr@dawarconsulting.com

  akansha@dawarconsulting.com