Pittsburgh, PA30+ days ago
Experience must include three (3) years working with: (i) Human immunology research, including primary human immune cell isolation, culture, stimulation, gene editing, and functional analysis; (ii) Murine models of inflammation, allergy, and atherogenesis, including designing and conducting in vivo experiments and processing a wide range of tissues (aorta, lungs, GI, skin) for immunologic analysis, histological analysis, and specialized staining (i.e. aortic plaque staining); (iii) Multiparameter flow cytometry, including experimental design, panel development, compensation, gating strategies, and data analysis; (iv) Molecular biology techniques, including nucleic acid extraction, qPCR, ddPCR, RNA-seq; (v) Gene-expression manipulation using lentiviral systems, including viral particles production and transduction for stable knockdown, knockout, or overexpression; (vi) Functional in vitro assays with mammalian cell lines, including evaluation of cell activation, viability, proliferation, cytokine production, etc; and (vii) handling, shaving, and injecting (IV, IP, intradermal) mice and processing broad range of tissues. Duties include: (i) independently leading scientific research projects and supervising trainees and research activities; (ii) High-level molecular biology, including sequencing and sample/library preparation; (iii) working with murine models of immune dysregulation, including asthma, neuroinflammation, autoinflammation, infection, and cardiovascular disease; (iv) immune cells functional assays; (v) cloning and transfection; and (vi) screening primary patient cells for disease-causing mutations.