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Chenega Professional & Technical Services, LLC., a Chenega Professional Services’ company, is looking for a Laboratory Technician 4. The Laboratory Technician shall provide laboratory support by assisting in the performance of aerosol exposures and animal research protocols as directed by AAT Leadership. Contractor shall independently assemble, calibrate, and test telemetry systems; troubleshoot electronic and software communication issues, and independently perform telemetry support for animal studies in BSL-2, BSL-3, and BSL-4 biocontainment laboratory environments.

<h4>Overview</h4><p>Come join a company that strives for <em>Extraordinary People and Exceptional Performance</em>! <strong>Chenega Professional & Technical Services, LLC.</strong>, a <strong>Chenega Professional Services</strong>’ company, is looking for a <strong>Laboratory Technician 4. </strong> The Laboratory Technician shall provide laboratory support by assisting in the performance of aerosol exposures and animal research protocols as directed by AAT Leadership.  The Contractor shall execute scientific studies, analyze data, and maintain laboratory space and equipment.</p><p>Our company offers employees the opportunity to join a team where there is a robust employee benefits program, management engagement, quality leadership, an atmosphere of teamwork, recognition for performance, and promotion opportunities. We actively strive to channel our highly engaged employee’s knowledge, critical thinking, and determination to innovate scalable solutions for our clients. </p><h4>Responsibilities</h4><ul><li>Generate, characterize and sample biological aerosols to support pathogenesis and medical countermeasure studies against biothreat agents. This shall include animal plethysmography, aerosol supervision and assisting, assaying, and decontaminations; attendance is required at scheduled trainings provided by qualified AAT staff. Workload is approximately 75-120 aerosol exposures per year.</li><li>Investigate independently, and in collaboration with others, events during the phases of infection in representative laboratory animals prior to and following agent exposure.  Support all technical aspects of animal protocols including but not limited to performing animal observations, physicals, sample collection, sample processing, and euthanasia as required; attendance is required at scheduled trainings provided by qualified Veterinary Medicine Division staff. All laboratory tasks shall be conducted under the guidance of a study PI and under the authority of the Institutional Animal Care and Use Committee (IACUC) and in compliance with GLP standards. Studies will be conducted in a BSL-2, BSL-3, or BSL-4 laboratory environment. Workload is up to 20 protocols per year. </li><li>Perform assays to include viral and bacterial stock characterization, plating assays, ELISA, RT-PCR, microarray, cytokine analysis, and applicable in vitro sample analysis assays as required by approved in vivo and in vitro protocols. </li><li>Perform clinical pathology evaluation such as hematology, clinical chemistry analysis, coagulation and similar, in support of approved animal protocols and NHP colony management operations.    </li><li>Maintain laboratory space and equipment. Record and document all equipment maintenance IAW USAMRIID SOPs and in compliance with GLP standards. </li><li>Assist with SOP writing, revision and review to meet GLP requirements and assist in the generation of SOP risk assessments. Contractor will appropriately respond to regulatory audits and Corrective and Preventative Actions (CAPAs) within the required timelines. Workload is typically ten (10) to fifteen (15) SOPs and five (5) to ten (10) CAPAs per year.</li><li>Contractor shall coordinate and collaborate with AAT leadership and personnel to assist in the development and continuation of the AAT technical training program. Contract staff will receive necessary training and once qualified by AAT leadership, assist in the training of other technical staff.</li><li>Contractor shall independently assemble, calibrate, and test telemetry systems; troubleshoot electronic and software communication issues, and independently perform telemetry support for animal studies in BSL-2, BSL-3, and BSL-4 biocontainment laboratory environments. Workload is approximately twenty (20) research studies per year.</li><li>Contractor shall operate and maintain the telemetry systems in a validated state for use in GLP animal studies. Approximately fifteen (15) to twenty-five (25) validated systems per year.</li><li>Shall maintain a safe and secure storage and backup of all original data files; tabulate and analyze telemetry data and provide results to Telemetry Support Subject Matter Expert (SME) for review as needed.</li><li>Shall maintain inventory of all telemetry system components to include hardware and software as needed.</li><li>Shall assist in the operation and maintenance of telemetry systems (e.g., DSI, Stellar and NOTOCORD). Systems will be maintained in a validated state as required for use in GLP animal studies; and assist in the maintenance and validation of telemetry equipment as needed.</li><li>Shall be trained in GLP and maintain records at that standard IAW all applicable regulations and USAMRIID SOPs.</li><li>Shall assist in the coordination and collaboration with scientists and technical staff within the Institute to provide telemetry services to include assisting in the training of study personnel on basic telemetry functions and data review.</li><li>Other duties as assigned.</li></ul><h4>Qualifications</h4><ul><li>Preferred Master’s degree or the equivalent in Molecular Biology, Microbiology, Biochemistry, Immunology, Biology, or a related field</li><li>Preferred 4 years’ experience in a bacteriology laboratory</li><li>Preferred prior experience in BSL-3 or BSL-4 laboratories</li><li>Preferred prior clinical diagnostics and regulatory laboratory experience</li><li>Preferred experience in BSL3 or BSL 4 environments</li><li>Preferred experience with animal inoculations, tissue collection and conduct bleeding</li><li>Preferred experience in basic cell culture, plaque assays, plaque reduction neutralization test, PCR, ELISA, Western Blot and clinical laboratory tests on animal samples</li><li>Prepare cloning plasmids, sequencing reactions and plasmid preparations</li><li>Experience injecting animals</li><li>Experience with animal gavage and blood collection</li><li>Prepared media</li><li>Experience with isolating and purifying DNA specimens</li><li>Process bacterial spore harvests</li><li>Ability to perform antibody determinations</li><li>Measure cytokines in cell cultures and cell extracts</li><li>Complete toxin neutralization assays</li><li>Attention to details; accurate record keeping; willingness to work in BSL-2 and BSL-3 high containment laboratories</li><li>Ability to plan, troubleshoot, and execute laboratory studies and equipment in a highly regulated environment</li><li>Ability to work in a matrixed, team-oriented environment involving multiple projects</li></ul><h4>Teleworking Permitted?</h4>Yes<h4>Teleworking Details</h4>Telework only permitted on due to base closure and/or inclement weather

<p>Chenega Corporation is a premier Government and Commercial services provider to the security, healthcare, IT, environmental, military operations, construction, and tourism industries. Our integral business practices are rooted in the Alaska native culture of our shareholders. We are known for our exceptional employees, our fiscal strength, our creativity and innovation, and for providing excellent cost and performance value to our customers and business partners. Chenega returns all its profit to its native shareholders</p>

Chenega Corporation

Research Associate IV - Aerosol

Aerosols

Analysis Skills

Animal Care

Animal Research

Antibodies

Assays

Bacteriology

Biochemistry

Biology

Blood Bank

Cell Cultures

Chemistry

Clinical Assessment

Clinical Laboratory

Clinical Pathology

Clinical Practices/Protocols

Cloning

Coagulation

Corrective Action

Cytokines

Data Analysis

Detail Oriented

Equipment Maintenance/Repair

Equipment Validation

GLP (Good Laboratory Practices)

Hardware Components

Hematology

Identify Issues

Immunology

Inventory Management

Laboratory

Laboratory Equipment

Laboratory Testing

Leadership

Medical Office Administration

Microbiology

Molecular Biology

Multitasking

Plating Processes

Polymerase Chain Reaction (PCR)

Professional Services

Record Keeping

Regulations

Regulatory Compliance

Research Protocols

Reverse Transcriptase PCR (RT-PCR)

Risk Analysis

Software Administration

Specimen Collection

Staff Training

Standard Operating Procedures (SOP)

System Validation

Systems Maintenance

Team Player

Technical Support

Telemetry

Training Program

Veterinary Medicine

Chenega

The Laboratory Technician 4 shall provide laboratory support with laboratory experiments in BSL-2 and BSL-3 laboratories and shall aid in the execution of scientific studies and plaque assays, PCR and other assays, animal procedures, collection/data analyzing, and maintain laboratory and laboratory equipment. Perform laboratory testing such as ELISA, Luminex Cytokine Analyses, acrophage phagocytosis assays, bacterial burdens in organ tissue, DNA chromosomal preparation, PCRs, isolating and purifying plasmid DNA specimens, and Agar gel analysis.

YEAR

<h4>Overview</h4><p>Come join a company that strives for <em>Extraordinary People and Exceptional Performance</em>! <strong>Chenega Professional Technical Services, LLC.</strong>, a <strong>Chenega Professional Services</strong>’ company, is looking for a <strong>Laboratory Technician 4 - Bacteriology Support. </strong> The Laboratory Technician 4 shall provide laboratory support with laboratory experiments in BSL-2 and BSL-3 laboratories and shall aid in the execution of scientific studies and plaque assays, PCR and other assays, animal procedures, collection/data analyzing, and maintain laboratory and laboratory equipment.</p><p>Our company offers employees the opportunity to join a team where there is a robust employee benefits program, management engagement, quality leadership, an atmosphere of teamwork, recognition for performance, and promotion opportunities. We actively strive to channel our highly engaged employee’s knowledge, critical thinking, and determination to innovate scalable solutions for our clients. </p><h4>Responsibilities</h4><ul><li>Assist Lead/PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence.  </li><li>Complete Opsonic Adherence assays to test the efficacy of animal sera (Rabbit and NHP) in the presence of Baby Rabbit Complement to adhere Ames bacilli and spores to RAW 264.7 macrophage cells.</li><li>Perform laboratory testing such as ELISA, Luminex Cytokine Analyses, acrophage phagocytosis assays, bacterial burdens in organ tissue, DNA chromosomal preparation, PCRs, isolating and purifying plasmid DNA specimens, and Agar gel analysis. </li></ul><ul><li>Prepare different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc). </li><li>FITC label and count Ames samples to check ratio of Bacilli: Spores over multiple time points. </li><li>Assist in the presentation of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to study investigator.  Use computer to prepare graphs and write reports for study investigator. </li></ul><ul><li>Perform Immunological and antibody determinations. </li><li>Perform Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression.  Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution. </li><li>Perform Cell Biology/Biochemical/microbiological tasks. Performs laboratory tests, such as protein gel electrophoresis, western blot analysis, growth of macrophage cell lines, live-dead cell determinations, prepares microbiological media for bacterial cultures. </li><li>Organize and maintain data generated from immunological and microbiological studies. </li><li>Assist in the performance animal challenges and bacterial challenge preparations to include preparing supplies and performing complex pathogenesis studies. </li><li>Perform animal injections, observations, gavages, blood collections, and necropsies. </li></ul><ul><li>Serve as a training representative for BSL-2 laboratory skills.</li><li>Order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment. Perform monthly Laboratory Management to include safety functions and inventory. </li></ul><ul><li>Review relevant literature monthly. Assist in the preparation standard operating procedures and manuscripts relating to their research efforts. Submit monthly reports to the TPOC. </li><li>Present research findings at one scientific conference per year. Present results in written and oral format publicly and internally as requested. </li><li>Other duties as assigned </li></ul><h4>Qualifications</h4><ul><li>Ability to meet BPRP and CS-PRP enrollment requirements</li><li>Ability to obtain and maintain Secret clearance</li><li>Demonstrated ability to perform laboratory and animal research tasks in regulated environments</li></ul><p><em>Preferred Qualifications:</em></p><ul><li>Master’s degree in Molecular Biology, Microbiology, Biochemistry, Immunology, Biology, or related field</li><li>4+ years of bacteriology laboratory experience</li><li>Experience working in BSL-3 or BSL-4 laboratories</li><li>Experience with clinical diagnostics and regulated laboratory environments</li></ul><p><em>Knowledge, Skills, and Abilities:</em></p><ul><li>Experience in BSL-2/BSL-3 laboratory environments</li><li>Proficiency with PCR, ELISA, flow cytometry, and microbiological assays</li><li>Experience with animal handling, inoculation, and sample collection</li><li>Ability to perform DNA isolation, cloning, and sequencing processes</li><li>Experience preparing laboratory media and reagents</li><li>Strong data recording, analysis, and reporting skills</li><li>Ability to execute studies in highly regulated environments</li><li>Attention to detail and strong organizational skills</li><li>Ability to work in team-based, multi-project environments</li><li>Ability to maintain security clearance and BPRP enrollments</li></ul><h4>Estimated Salary/Wage</h4>USD $65,000.00/Yr. Up to USD $75,000.00/Yr.

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Research Associate IV - Aerosol Chenega Corporation

Laboratory Technician 4 - Bacteriology Support Chenega Corporation

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