The vast majority of work will be tissue culture (cell culture maintenance, generating stable cells lines, primary neuronal cultures, stem cells including iPSCs); transfection in cellular studies; molecular cloning; RNA, DNA and protein extraction and analysis (PCR, RT-PCR, western blot); tissue collection from mice; immunocytochemistry and immunohistochemistry handling human tissue and in vitro organoids for genomic analysis including, but not limited to, single-cell transcriptomics and epigenomics, immunohistochemistry, and In Situ RNA hybridization. Furthermore, the SRA II will be involved in transformation and generating DNA plasmids; performing PCR and gel electrophoresis; will be involved in generating spatial transcriptomics, immunocytochemistry and immunohistochemistry, preparing buffers, solutions, and chemicals for lab experiments; basic data analysis and interpretation; maintaining database of experimental results; assisting with figures for grant applications and publications; providing technical assistance in the lab, including assisting other members of the lab; and assisting with training of lab assistants and students.