Histotechnologist / Spatial Biology Specialist

Job ID: 37804746

Location: Ridgefield, CT
Client: Boehringer Ingelheim
Department: Oncology Research – Discovery Research
Duration: 12 Months
Work Arrangement: On-Site
Job Type: Contract

Job Description

Sigma, Inc. is seeking an experienced Histotechnologist / Spatial Biology Specialist to join the Oncology Research – Discovery Research team at Boehringer Ingelheim in Ridgefield, CT.

This is an exciting opportunity for a laboratory professional with a strong background in histology, tissue processing, immunohistochemistry (IHC), immunofluorescence (IF), and advanced tissue-based assays to contribute to cutting-edge oncology and translational biomarker research.

The selected candidate will help develop and expand spatial biology capabilities, supporting preclinical and translational research through high-plex protein imaging, spatial transcriptomics, and quantitative image analysis.

We are particularly interested in candidates who combine hands-on laboratory expertise with imaging and data analysis skills and are interested in advancing innovative spatial biology technologies.

Key Responsibilities

Spatial Biology & Assay Development

• Develop, optimize, and perform high-plex tissue-based assays, including multiplex immunofluorescence and spatial protein profiling.

• Design and optimize multiplex antibody panels, including antibody selection, staining protocols, controls, and assay validation.

• Support spatial transcriptomics and emerging spatial gene-expression technologies.

• Develop integrated workflows involving tissue preparation, staining, imaging, and quantitative analysis.

• Perform tissue annotation, cell segmentation, phenotyping, spatial quantification, and image visualization.

• Collaborate with computational scientists to integrate spatial protein, RNA, imaging, and histopathology data.

Histology & Tissue Processing

• Perform tissue processing, embedding, microtome sectioning, slide preparation, H&E staining, IHC, IF, ISH, and coverslipping.

• Work with human and mouse FFPE and frozen tissue samples.

• Optimize tissue preparation and sectioning techniques for advanced spatial biology assays.

• Perform quality control of tissue sections, staining procedures, and imaging results.

Laboratory & Research Support

• Operate laboratory instrumentation used for histology, multiplex staining, imaging, and slide scanning.

• Maintain accurate laboratory records, experimental protocols, SOPs, and sample tracking documentation.

• Troubleshoot assay workflows and improve reproducibility and data quality.

• Collaborate with multidisciplinary teams across pathology, oncology, molecular biology, translational research, and computational sciences.

Required Qualifications

• Bachelor's or Master's degree in Biology, Histotechnology, Pathology, Biomedical Sciences, or a related discipline.

• Hands-on laboratory experience in histology, tissue processing, or tissue-based research.

• Knowledge of tissue preparation, staining, and laboratory quality control procedures.

• Strong attention to detail and ability to maintain accurate experimental documentation.

• Excellent communication and collaboration skills.

Preferred Qualifications

Candidates do not need to possess every qualification listed below. Applicants with complementary wet-lab and image analysis experience are particularly encouraged to apply.

• Experience with multiplex immunofluorescence (mIF) and high-plex protein imaging.

• Experience with antibody panel development and optimization.

• Hands-on experience with IHC, IF, ISH, H&E staining, and tissue sectioning.

• Experience working with human and mouse FFPE and frozen tissues.

• Familiarity with spatial transcriptomics or spatial gene-expression technologies.

• Experience with quantitative microscopy and high-content tissue imaging.

• Knowledge of image analysis techniques, including tissue annotation, cell segmentation, phenotyping, and spatial measurements.

• Familiarity with R, Python, or other data analysis tools.