Laboratory Research Technician I- Kuhn Lab

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Laboratory Research Technician I - Kuhn Laboratory

The University of Pittsburgh, Department of Immunology, is seeking a full-time technician to support newly funded research programs. The Kuhn lab is embedded in the Center for Systems Immunology (CSI) and investigates cell-cell communication in tumor malignancy to uncover fundamental insights into how the tumor microenvironment is assembled on a molecular and cellular level. We develop and apply functional genomics tools to genetically engineer and analyze cancer-associated fibroblasts (CAFs) and immune cells in tumors The goal is to identify novel approaches for improved diagnostics and cell therapies in cancer.

The applicant will support the function of the laboratory and assist the supervisor conducting experiments in organismal, cellular, and molecular tumor immunology, with direct supervision. The applicant must be comfortable closely working with a small team of scientists, comfortable handling laboratory mice as part of their research, and eager to learn new laboratory techniques and apply them carefully and accurately.

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Required skills: Interest in organismal biological sciences. Interest in conducting both wet-lab experiments and computational analysis and coding. Previous experience with basic wet-lab procedures (pipetting, liquid handling, calculation of dilutions/ratios/concentrations of reagents). Demonstrate problem solving and troubleshooting skills, with an understanding how to seek help. Strong organizational and planning skills, with a conscientious attention to detail. Must keep detailed and accurate records.

Desired skills: Basic programming skills in R and/or Python; basic understanding and experience in flow cytometry, immunofluorescence microscopy, sterile cell culture.

Qualifications: A bachelor's degree in biology, immunology or related field is required. Knowledge of mathematical and chemical principles. Must be highly organized and efficient with their time. Must maintain professional demeanor and work well with others. Must be able to prioritize daily duties.

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Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

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Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

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Perform supervised laboratory work/experiments, including guided analysis of cell culture and mouse experiments; gene engineering

Write reports and summaries of findings with guidance

work includes molecular biology, cell isolation from mouse organs, sterile cell culture, viral production for gene engineering, flow cytometry, cell sorting, immunofluorescence

Maintain and oversee lab inventories and lab work areas with guidance

Maintain mouse colony

Assist supervisor and other lab members in laboratory procedures

Work with supervisor to prepare data for presentation to the group and for publication

Work with supervisor to prepare data for presentation to the group and for publication

'',''!*!

Perform supervised laboratory work/experiments, including guided analysis of cell culture and mouse experiments; gene engineering

Write reports and summaries of findings with guidance

work includes molecular biology, cell isolation from mouse organs, sterile cell culture, viral production for gene engineering, flow cytometry, cell sorting, immunofluorescence

Maintain and oversee lab inventories and lab work areas with guidance

Maintain mouse colony

Assist supervisor and other lab members in laboratory procedures

Work with supervisor to prepare data for presentation to the group and for publication

Work with supervisor to prepare data for presentation to the group and for publication

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"must be able to sit or stand for prolonged periods of time."

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"must be able to sit or stand for prolonged periods of time."

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The University of Pittsburgh is an equal opportunity employer / disability / veteran.

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