Key Responsibilities include:
Develop expertise with multiple gene editing tools allowing knock-in, knock-out, silencing and other genetic manipulation activities
Perform wet lab activities related to engineering of mammalian and non-mammalian animal cells
Perform flow cytometry
Design, produce, and test novel gene editing constructs using molecular cloning and other techniques based on robust, well-controlled scientific experiments (including plasmid digestion, purification, ligation, transformation, and sequencing)
Perform In vitro transcription to produce RNA from DNA templates
Perform DNA/RNA extraction and downstream analytical assays such as qPCR and RT-qPCR
Assist in performance of primary and iPSC-derived cell culture, transfection, and cell-based assays
Execute and troubleshoot standard protocols, develop and adapt new protocols, and query literature to incorporate additional assays as needed
Independently plan and execute experiments
Document all experimental planning, progress, experimental data and genetic designs in electronic lab notebook, present research design and results to multi-disciplinary teams and leadership
Create experimental workflows and collect quantitative measurements for engineered cells including flow cytometry and multi-omics techniques
Analyze and distill insight from complex molecular data streams
Collaborate frequently with computational biologists, protein engineers and cell biologists to incorporate biological design principles and experimental datasets into the synthetic biology product development pipeline
Test and characterize molecular and cellular system targets using biochemistry, molecular biology and/or genetic approaches
Leverage knowledge of gene editing technologies to contribute scientifically to goals and strategy
The ideal candidate will possess the following qualities & qualifications:
Ph.D. in Biological Engineering, Synthetic Biology, or a related field + 3 years of experience doing research in an academic or industry lab setting, M.S. + 10 years of experience in related field , or B.S. + 13 years of experience in related field
Skilled in molecular biology and cell culture techniques
Ability to work both independently and in a collaborative team environment
Quantitative data analysis expertise
Demonstrated experience in design, analysis and interpretation of in vitro and cell- based assays to assess on and off-target gene editing events using NGS methods (short and long-read) and ddPCR
Track record of scientific innovation and thinking outside-of-the-box
Ability to communicate complex scientific issues in a logical and coherent manner
Experience in flow cytometry, -omics, and analytical techniques a plus
Experience with bioproduction and GMP/GLP regulation a plus
Experience with gene editing techniques a plus
| Location | Cambridge, Massachusetts |