Research Assistant I - Cellular Therapy

Job Description

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Job Title: Research Assistant I - Cellular Therapy

Division: Pediatrics

Work Arrangement: Onsite only

Location: Houston, TX

Salary Range: $47,372 - $55,371

FLSA Status: Nonexempt

Work Schedule: Monday - Friday, 8 a.m. - 5 p.m.

Summary

A Research Assistant I position is available at the Center for Advanced Innate Cell Therapy within the Texas Children's Cancer Center under the supervision of Dr. Gengwen Tian. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. Duties include blood and tissue sample processing, cell culture, molecular cloning, PCR, gene transduction, flow cytometry, in-vitro functional assays of immune effector cells, and measuring anti-tumor activity in mice. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment. Candidates must have at least a Bachelor''s degree in Cell Biology, Molecular Biology or Biochemistry and a prior hands-on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice.

Job Duties

• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Performs of expansion of lymphocytes in culture.
• Performs retroviral/lentiviral transduction of cultured cells.
• Performs CRISPR knockout on NKT and T cells.
• Performs immunoflourescent staining and runs and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo software.
• Perform T or NKT cell culture and measure their antitumor activity
• Measure cytokine production using ELISA or Luminex assays
• Perform mouse experiments, such as IV injection, IP injection and luminescent imaging.
• Documents results of experiments and prepare reports
• Performs molecular cloning and PCR
• Keep laboratory records
• Generates reports summarizing experimental result
• Performs other job related duties as assigned.

Minimum Qualifications

• Bachelor''s degree in a Basic Science or a related field.
• Two years of relevant experience.

Preferred Qualifications

• Master''s degree in basic science or a related field.

Work Authorization Requirement:

This position is not eligible for visa sponsorship. Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

Requisition ID: 26620

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