Research Technician

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The Department of Structural Biology at the University of Pittsburgh School of Medicine is located in Biomedical Science Tower 3. We currently have twelve faculty members working in the fields of cryo-electron microscopy, NMR spectroscopy, X-ray crystallography, and biochemistry. This laboratory technician position requires a B.S. degree in a relevant field of study.

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Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

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Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

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Conducting and managing laboratory experiments. Manage multiple research projects concurrently using techniques such as PCR, DNA cloning, sample preparation for electron microscopy, and cryo-EM data collection/analysis. Work together with PI or independently to perform complex laboratory work and technical analysis. Troubleshoot technical problems and find suitable experimental solutions or design new procedures to circumvent problems.

Research concentrates on trafficking essential for cilia or flagella biogenesis using cryogenic electron tomography. By combining cell biology and structural biology, experiments aim to understand molecular and structural function cilia or flagella using Trypanosoma brucei, a single celled parasite cell, as a model organism and pave the way to treat various important ciliopathy diseases. The successful candidate will work with other lab members to conduct cell culture, molecular and cellular biology (cloning, genetic manipulation of singe-cell system), immunofluorescence-labelling, and cryo-electron tomography. Organization of lab space, coordination with other lab members for ordering and stocks, and presentation of data at lab meetings are essential. The candidate will assist the PI with experimental design, implementation of novel strategies/procedures, and manuscript preparation for publication, including figures and writing.

The applicant should be highly motivated, well-organized, detail-oriented, be able to prioritize multiple tasks, and will be encouraged to work independently. Strong interpersonal skills and judgment is essential. The successful applicant should have some experience in molecular biology including PCR, cloning, or SDS-PAGE, or fluorescent imaging, and cell culture. Experience in cryo-EM (electron microscopy, FIB milling) and proficient computer skills (Linux, python, scripting) are highly desired but not essential. Most of all we are looking for applicants that are motivated to learn novel technologies, take on new challenges, and to develop innovative techniques and methods.

'',''!*!

Conducting and managing laboratory experiments. Manage multiple research projects concurrently using techniques such as PCR, DNA cloning, sample preparation for electron microscopy, and cryo-EM data collection/analysis. Work together with PI or independently to perform complex laboratory work and technical analysis. Troubleshoot technical problems and find suitable experimental solutions or design new procedures to circumvent problems.

Research concentrates on trafficking essential for cilia or flagella biogenesis using cryogenic electron tomography. By combining cell biology and structural biology, experiments aim to understand molecular and structural function cilia or flagella using Trypanosoma brucei, a single celled parasite cell, as a model organism and pave the way to treat various important ciliopathy diseases. The successful candidate will work with other lab members to conduct cell culture, molecular and cellular biology (cloning, genetic manipulation of singe-cell system), immunofluorescence-labelling, and cryo-electron tomography. Organization of lab space, coordination with other lab members for ordering and stocks, and presentation of data at lab meetings are essential. The candidate will assist the PI with experimental design, implementation of novel strategies/procedures, and manuscript preparation for publication, including figures and writing.

The applicant should be highly motivated, well-organized, detail-oriented, be able to prioritize multiple tasks, and will be encouraged to work independently. Strong interpersonal skills and judgment is essential. The successful applicant should have some experience in molecular biology including PCR, cloning, or SDS-PAGE, or fluorescent imaging, and cell culture. Experience in cryo-EM (electron microscopy, FIB milling) and proficient computer skills (Linux, python, scripting) are highly desired but not essential. Most of all we are looking for applicants that are motivated to learn novel technologies, take on new challenges, and to develop innovative techniques and methods.

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Ability to manipulate laboratory instruments and reagents to conduct experiments; ability to move about the lab environment. Will require standing or sitting at lab benches for long periods of time.

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Ability to manipulate laboratory instruments and reagents to conduct experiments; ability to move about the lab environment. Will require standing or sitting at lab benches for long periods of time.

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The University of Pittsburgh is an equal opportunity employer / disability / veteran.

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