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Biotechnology Jobs in South San Francisco, CA

Scientist II

ICONMA, LLC
  • South San Francisco, CA
  • $49.28–$65.65 Per Hour
  • Instant Apply
10 days ago

Job Description

Our Client, a Biotech company, is looking for a Scientist II for their South San Francisco, CA location.
 
Responsibilities:
  • Integration of automation and high-throughput LVV and AAV workflows. Implement and program automated liquid handling systems (e.g., Integra Assist Plus, Bravo etc) for high-throughput transfection, transduction, sample dilution and cell seeding. Incorporate high-throughput shaker arrays or automated micro-scale bioreactor platforms (e.g., Ambr® 15 or Ambr® 250) to run parallel mini-scale expression runs. Troubleshoot liquid-class parameters, pipetting precision, and plasticware compatibility to minimize batch-to-batch variation.
  • Develop and optimize suspension-based lentiviral vector (LVV) upstream production platform.
  • Design, execute, and characterize robust, scalable upstream processes, from seed train expansion through shake flask production and primary harvest.
  • Apply Design of Experiments (DOE) methodologies to systematically explore multivariate interactions including multi-plasmid transient transfection conditions, plasmid ratios, transfection reagents (e.g., PEI, lipid nanoparticles), DNA-to-cell ratios, harvest timing, feed schedule and chemical enhancers to boost packaging efficiency.
  • Development and establishment of downstream LVV process.
  • Evaluate and identify optimal low-shear depth filtration and microfiltration protocols tailored to large scale virus production.
  • Develop and optimize ultrafiltration/diafiltration (TFF) and chromatographic purification methodologies.
  • Conduct cutting-edge analytics for LVV and AAV QC.
  • Perform physical and functional characterization of viral vectors to interpret quality attributes and ensure high-quality services.
  • Integrate QC in the uptream/downstream process development for rapid identification of factors for improvement.
  • Data, Inventory and Protocol Management: Analyze multivariate process datasets using excel, Prism or AI agent to facilitate fast progression of projects.
  • Maintain a meticulously organized virus inventory and registry utilizing digital informatics platforms to ensure data integrity and traceability.
  • Author standardoperating procedures (SOPs), technical reports and tech transfer protocols.
  • Present data in group and department meetings.
 
Requirements:
  • Proficient in mammalian cell culture and general molecular biology techniques such as transient plasmid transfection, PCR, qPCR, ddPCR, dPCR and Western blot.
  • Require strong data analysis and presentation skills.
  • Previous experience with ex vivo CAR T engineering using lentivirus is a must.
  • Working experiences with lentivirus or AAV production in adherent or suspension cells, process development, statistical DOE modeling and automated liquid handling are highly desirable.
  • Experience with flow cytometer operations, electronic notebook and BSL2 facility management are also strong plus.
  • Must be self-motivated, detail-oriented, analytical, highly organized, and have excellent communication and interpersonal skills.
  • MS/PhD degree in Cell Biology, Molecular Biology, Virology, Biochemistry, Bioengineering, Genetics, or a related discipline with 3+ years of laboratory research or industrial experience.
 
Why Should You Apply?Why Should You Apply?

Numbers & Facts

LocationSouth San Francisco, CA
Salary$49.28–$65.65 Per Hour

Skills

  • Analysis Skillsunmatched
  • Artificial Intelligence (AI)unmatched
  • Automationunmatched
  • Automotive Engineeringunmatched
  • Biochemistryunmatched
  • Bioengineeringunmatched
  • Biotech and Pharmaceuticalunmatched
  • Cell Biologyunmatched
  • Cell Culturesunmatched
  • Chromatographyunmatched
  • Communication Skillsunmatched
  • DNAunmatched
  • Data Analysisunmatched
  • Data Qualityunmatched
  • Data Setsunmatched
  • Detail Orientedunmatched
  • Digital Polymerase Chain Reaction (Digital PCR)unmatched
  • Droplet Digital PCR (ddPCR)unmatched
  • Experiment Designunmatched
  • Facilities Managementunmatched
  • Geneticsunmatched
  • Health Planunmatched
  • High Throughputunmatched
  • Identify Issuesunmatched
  • Industrial Researchunmatched
  • Informaticsunmatched
  • Interpersonal Skillsunmatched
  • Inventory Managementunmatched
  • Molecular Biologyunmatched
  • Multivariate Analysisunmatched
  • Organizational Skillsunmatched
  • Polymerase Chain Reaction (PCR)unmatched
  • Presentation/Verbal Skillsunmatched
  • Prismunmatched
  • Procedure Developmentunmatched
  • Process Analysisunmatched
  • Process Developmentunmatched
  • Protocol Analysisunmatched
  • Quality Controlunmatched
  • Real-time PCR (qPCR)unmatched
  • Research Laboratoryunmatched
  • Standard Operating Procedures (SOP)unmatched
  • Statistical Modelingunmatched
  • Traceabilityunmatched
  • United States Department of Energy (DOE)unmatched
  • Vector Basedunmatched
  • Virologyunmatched
  • Virusesunmatched

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