San Francisco, CA3 days ago
The individual will set up and perform novel experiments and molecular-cellular techniques necessary for research projects of the lab such as molecular cloning, DNA/RNA isolation, stem cell differentiation, transfection, lenti- or adeno-associated virus infection of cells, CRISPR-Cas9 gene editing, cell imaging (live and fixed/stained), cell-based luciferase assys, genomic profiling assays including RNA-seqm ATAC-seq, and ChIP-Seq, and DNA-protein binding assays including EMSA. Responsibilities will include some of all of the following (as needed for the project): performing or assisting with stem cell differentiation and maintenance, molecular biology assays, performing mouse surgeries to implant transmitters, managing a mouse colony, imaging using confocal and light sheet microscopy, preparation of libraries for high throughput sequencing, and general lab maintenance.