Hands-on experience measuring transport across tight-junction barrier models in vitro/ in• vivo, and an understanding of receptor-mediated transcytosis, uptake, and intracellular• trafficking Proven tissue culture experience with human cell lines and primary human cells A track record building novel cell-based assays that moved a drug discovery program• forward Practical expertise in nucleic acid assays (qPCR, RNA-seq) and protein-based assays(flow cytometry, ELISA, Western blotting, high-content imaging), including DNA and• RNA purification Scientific independence: owning a project, shaping its research plan, and becoming the• internal expert on new targets and disease biology quickly. Develop and execute new cell-based assays to screen constructs against multiple• targets, including transport across in vitro barrier models (transwell systems, primary and• iPSC-derived cells, co-culture) Develop, optimize, and perform cell-based assays to evaluate the pharmacology of• binders and AOC variants on a variety of phenotypic readouts including receptor binding,• trafficking and transcytosis Culture and differentiate multiple cell lines and primary human cells using established• and custom protocols Work within an integrated, cross-functional team to lead candidate optimization using in• vitro and in vivo models to advance the program towards key decision points.