Execute full bioanalytical method validation to ICH M10 — selectivity, specificity, calibration curve and range, within- and between-run accuracy and precision, matrix effect and recovery, carryover, dilution integrity, and the complete stability panel (stock/working solution, benchtop, freeze-thaw, processed/autosampler, and long-term matrix stability). Address peptide-specific bioanalytical challenges, including non-specific/adsorptive loss, protease-driven instability, and quantitation of endogenous or endogenous-like peptides (e.g., thymosin 4-class analytes) requiring surrogate-matrix or authentic-matrix background-subtraction approaches.