Cell Culture: derivation, culture, characterization and differentiation of pluripotent stem cells, day to day maintenance of cell cultures, freeze and thawing cell cultures, maintain cell culture records, order reagents, supplies and media; make reagent solutions, culture media; routine equipment maintenance, laboratory analysis by molecular, histological, and immunocytochemical methods. Provide assistance in studies on mitochondrial biology and epigenetic and genetic signatures of pluripotent stem cells, DNA, RNA and protein sample processing, preparation of media, general sterile cell culture laboratory upkeep, (manual and computerized).