Research activities will include induction of osteoarthritis in a rat model, intra-articular administration of hydrogel therapeutics, longitudinal monitoring of treatment response, collection and processing of biological samples, assessment of local and systemic inflammatory responses, and comprehensive tissue characterization through histological, immunohistochemical, and molecular analyses of joint tissues. The projects include synthesis of bioinks/bioresins, volumetric bioprinting (volumetric additive manufacturing; VAM), oxygen measurements, physiochemical biomaterial characterization, stem cell culture, isolation of human cells and fluids, multiplexed proteomics, biological characterization of cell-laden hydrogels (e.g.