Experience with methods such as qPCR, ddPCR, RNA-seq, western blot, ELISA, reporter assays, splice assays, cytokine panels, or tissue analysis is helpful; expertise in every method is not required. Select oligonucleotide chemistries—including phosphorothioate backbones, 2′-MOE, 2′-OMe, LNA, cEt, PMO, and GalNAc or lipid conjugation—based on tissue, mechanism, potency, durability, safety, off-target risk, immune activation, and manufacturability.