Studies include dissection of embryonic and postnatal mouse brain regions; tissue fixation, embedding, and cryosectioning; brain tissue antigen retrieval, histochemical and immunofluorescent identification of protein expression; embryonic and postnatal mouse brain neuroanatomy, with focus on cerebral cortex, hippocampus, cerebellum; computer-based tissue and cell quantitative analysis including NeuroStereology; brightfield and ultraviolet microscopy; data acquisition and entry into Excel spreadsheets and application of GraphPad software; preparation of graphs and PowerPoint slide presentations; performance of immunoblotting techniques of brain homogenates, and PCR analysis of genotypes; literature search and summary. Aligned with Rutgers University-New Brunswick and collaborating university wide, RBHS includes eight schools, a behavioral health network, and six centers and institutes that focus on cancer treatment and research, neuroscience, clinical and translational research, advanced biotechnology and medicine, environmental and occupational health, and health care policy and aging research.