Develop, optimize, and execute robust in vitro assays (using Flow Cytometry/FACS, plate readers, and fluorescence/luminescence imaging) to evaluate target binding, receptor-mediated cellular uptake, endosomal escape, and functional cargo translation. Design and execute tLNP formulations encapsulating nucleic acid payloads (e.g., mRNA, sgRNA), systematically altering lipid ratios, ionizable lipid structures, and helper components to drive extrahepatic tissue tropism.